Review



methyl-cpg binding protein mbd2b  (Active Motif)


Bioz Verified Symbol Active Motif is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Active Motif methyl-cpg binding protein mbd2b
    A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with <t>MBD2b,</t> a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).
    Methyl Cpg Binding Protein Mbd2b, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methyl-cpg+binding+protein+mbd2b/pmc04021823-165-5-10?v=Active+Motif
    Average 90 stars, based on 1 article reviews
    methyl-cpg binding protein mbd2b - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Developmental Epigenetics of the Murine Secondary Palate"

    Article Title: Developmental Epigenetics of the Murine Secondary Palate

    Journal: ILAR Journal

    doi: 10.1093/ilar.53.3-4.240

    A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).
    Figure Legend Snippet: A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).

    Techniques Used: Methylation, Isolation, Sonication, Incubation, Binding Assay, Amplification, Whole Genome Amplification, Control, Labeling, Hybridization, Microarray



    Similar Products

    methyl-cpg binding protein mbd2b  (Active Motif)
    90
    Active Motif methyl-cpg binding protein mbd2b
    A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with <t>MBD2b,</t> a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).
    Methyl Cpg Binding Protein Mbd2b, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methyl-cpg+binding+protein+mbd2b/pmc04021823-165-5-10?v=Active+Motif
    Average 90 stars, based on 1 article reviews
    methyl-cpg binding protein mbd2b - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).

    Journal: ILAR Journal

    Article Title: Developmental Epigenetics of the Murine Secondary Palate

    doi: 10.1093/ilar.53.3-4.240

    Figure Lengend Snippet: A schematic outline to identify differentially methylated genes using promoter microarrays. Genomic DNAs from secondary palates (gestation days 12–14) were isolated and fragmented by sonication. Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments. The enriched DNA fragments from all three gestational days were amplified by whole genome amplification. Genomic DNAs not subjected to methylation enrichment were used as controls. Control DNA, which was labeled with Cy3, and experimental DNA, which was labeled with Cy5, were hybridized to NimbleGen 2.1 M mouse promoter microarrays (Roche NimbleGen, Madison, WI). Nine microarrays representing three biologic replicates on each of gestation days 12, 13, and 14 were probed. After hybridization and washing, the microarray chips were scanned and the accrued data were analyzed using biostatistical methods (Seelan et al., unpublished data).

    Article Snippet: Sonicated DNA was incubated with MBD2b, a methyl-CpG binding protein (Active Motif, Inc., Carlsbad, CA), for enrichment of methylated genomic fragments.

    Techniques: Methylation, Isolation, Sonication, Incubation, Binding Assay, Amplification, Whole Genome Amplification, Control, Labeling, Hybridization, Microarray